ABSTRACT
Objective: To investigate the association between early growth response gene 1 (EGR1) and Alzheimer's disease (AD) in Han Chinese people. Methods: A total of 715 AD patients and 760 health controls were recruited in two independent samples from Eastern China (382 AD patients and 426 normal individuals) and Southwest China (333 AD patients and 334 normal individuals). SNaPshot technique was utilized to analyse the single nucleotide polymorphism (SNP) of rs11743810. A public database was used to explore whether EGR1 gene was differentially expressed in the brain of AD patients and health controls. Then the protein-protein interaction (PPI) assessment was conducted using the STRING database, and the brain eQTL (expression quantitative trait loci) analysis was used to explore the difference in rs11743810 expression between different genotypes in different brain regions. Results: Cross-platform normalized data showed that there was significant difference of EGR1 expression in temporal cortex between AD patients and control subjects (|log2FC|=0.780, P=0.000 before FDR corrected; P=0.001 after FDR corrected). PPI analysis revealed that EGR1 was physically connected with amyloid precursor protein (APP) and clusterin (CLU) protein in the network. However, different genotypes of rs11743810 showed no significant difference in expression in 10 brain regions, and no significant difference in the genotype and allele frequency of rs11743810 between AD patients and controls were found in our two independent samples. Conclusion: The rs11743810 in EGR1 may not be major susceptibility gene site for AD in Han Chinese people.
ABSTRACT
Objective To investigate the chemical constituents from the ethanol extract of Ulva pertusa. Methods The compounds were isolated and purified by chromatography on silica gel, ODS, Diaion HP-20, Sephadex LH-20, and preparative HPLC methods. Their structures were elucidated on the basis of spectral data. Results Eighteen compounds were isolated and identified as isophitol (1), indole-3-carboxylic acid (2), 1-O-palmitoyl-3-O-(6'-sulfo-α-D-quinovopyranosyl) glycerol (3), (2S)-1-O-palmitoyl-3-O-[α-D- galactopyranosyl-(1→2)-β-D-galactopyranosyl] glycerol (4), 3-methylsulfoxypropionic acid (5), 3-chloropropionic acid (6), tyrosol (7), 4-hydroxybenzoic acid (8), 4-hydroxyphenylacetic acid (9), 6-vinyl hexanolide (10), loliolide (11), annuionone D (12), azelaic acid (13), succinic acid (14), 8-hydroxy-(6E)-octenoic acid (15), 3-ethoxypropionic acid (16), n-butyl β-D-fructopyranoside (17), and n-butyl pyroglutamate (18). Conclusion Compounds 1-16 are isolated from this alga for the first time, and compounds 5, 6, 10, 15, and 16 are obtained from natural products for the first time. Compounds 17 and 18 are artifacts of isolation from β-D-fructopyranoside and pyroglutamic acid.